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KMID : 0545120020120030444
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.444 ~ p.448
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High Cell Density Cultivation of Bifidobacterium longum Using a Calcium Carbonate-Alginate Beads System
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Yu, Won Kyu
Kim, Ji Youn/Lee, Ki Yong/Heo, Tae Ryeon
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Abstract
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A CaCO_3-alginate beads system was developed for high cell density cultivation of Bifidobacterium longum and the cost-effective media were also screened. In batch process with CaCO_3 beads, two strains of B. longum showed both the highest viable cells and optical density in TPY medium, resulting in maximum optical density and viable cell counts of 12.40, 2.22×10 exp (10) cfu/ml for B. longum ATCC 15707 and 13.71, 3.93×10 exp (10) cfu/ml for B. longum HLC 3742. Released size distribution, according to CaCO_3-alginate bead size preparation, was smaller than others. These results were also examined by observing their morphology. The skim milk-based medium was most adequate to cultivate B. longum as the cheapest medium, and 10% skim milk supplemented with 2% glucose and 1% yeast extract was a suitable medium, supporting the growth to 5.57×10 exp (10) cfu/ml for ATCC 15707 and 6.82×10 exp (9) cfu/ml for HLC 3742. During the long-term storage at 4℃ and -20℃, B. longum cultivated with CaCO_3 beads had the highest stability. Consequently, CaCO_3-alginate beads buffer was found to be useful not only to cultivate B. longum but also to preserve cultures.
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